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Image Search Results
Journal: Frontiers in Physiology
Article Title: Drivers of Sinoatrial Node Automaticity in Zebrafish: Comparison With Mechanisms of Mammalian Pacemaker Function
doi: 10.3389/fphys.2022.818122
Figure Lengend Snippet: Mechanisms of zebrafish sinoatrial node (SAN) automaticity. Effects of pharmacological block of membrane (40 min of 3 μM ivabradine hydrochloride for intracellular block of “funny” current, I f , n = 7; 7 min of 3 mM cesium chloride for extracellular block of I f , n = 6; 15 min of 165 μM nickel(II) chloride for block of T-type Ca 2+ current, I Ca,T , n = 7) or Ca 2+ (15 min of 1 μM ryanodine for block of ryanodine receptors, n = 6; 30 min exposure of 5 μM BAPTA-AM, followed by 30 min to allow for de-esterification of the AM group, for chelation of cytosolic Ca 2+ , n = 7; 15 min of 0.2 μM nifedipine for block of L-type Ca 2+ current, I Ca,L , n = 6) “clock” components, as well as matched time controls (60 min, n = 7) on heart rate (A,C,E) and the inter-beat variability of cycle length (measured as the root mean square of its successive differences, RMSSD CL ; B , D , F ), in the isolated zebrafish heart. Representative electrocardiogram (ECG) recording of zebrafish cardiac rhythm in control and after combined pharmacological block of membrane and Ca 2+ “clock” components (3 mM cesium chloride, 165 μM nickel(II) chloride, and 1 μM ryanodine, n = 6) and measurements of the resulting SAN pause and burst duration and control and burst heart rate (G) . Data shown as mean ± SEM. Means compared by paired, two-tailed, Student’s t -tests; * p < 0.05.
Article Snippet: The electrodes were connected to an
Techniques: Blocking Assay, Isolation, Two Tailed Test
Journal: Frontiers in Physiology
Article Title: Drivers of Sinoatrial Node Automaticity in Zebrafish: Comparison With Mechanisms of Mammalian Pacemaker Function
doi: 10.3389/fphys.2022.818122
Figure Lengend Snippet: Effect of mechanical preload on zebrafish and rabbit sinoatrial node automaticity. Representative electrocardiogram (ECG) recording of zebrafish (A) and rabbit (D) sinoatrial node rhythm under unloaded (slack) or mechanically preloaded (5 mg or 300 mg) conditions. Heart rate over time of individual zebrafish ( B , n = 8) and rabbit ( E , n = 8) sinoatrial nodes when mechanically unloaded or preloaded. Average heart rate and the inter-beat variability of cycle length (measured as the root mean square of its successive differences, RMSSD CL ) of the zebrafish (C) and rabbit (F) sinoatrial node when mechanically unloaded or preloaded. Data shown as mean ± SEM. Means compared by paired, two-tailed, Student’s t -tests; * p < 0.05.
Article Snippet: The electrodes were connected to an
Techniques: Two Tailed Test